LONZA人表皮角质形成细胞
中文名称：正常成人表皮角质形成细胞
英文名称：NHEK-Ad - Human Epidermal Keratinocytes, Adult, Single Donor
产品货号：00192627
1 x Cryopreserved ampule of NHEK-Ad from single donor containing ≥ 500,000 cells
Product Overview
Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysi is provided for each cell lot purchased.
Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 00192627B) and get a discounted price on cells and media.The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 00192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 00192060)
中文名称：新生儿表皮角质形成细胞-多供体混合
英文名称：NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
产品货号：00192906
Cryopreserved Human Neonatal Epidermal Keratinocytes, Pooled, ≥ 500,000 cells
Product Overview
Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult and neonatal cells.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis |is provided for each cell lot purchased.
References with Lonza’s Keratinocytes:
Protective barrier:
Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M, Mlitz V, Gruber F, Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E. Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47.
Skin model:
Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207.
Cancer:
Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS. EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71.
Human Papillomaviruses (HPV) mediated skin cancer response:
An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients. The present study utilized pooled keratinocytes cultured in KGMTM-Gold Medium from several healthy donors. The data showed that betaPV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165.
LONZA人表皮角质形成细胞说明：
长期供应人表皮角质形成细胞
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——LONZA中国一级代理商，北京泽平，长期供应LONZA原代细胞