Pall Xpansion贴壁细胞生物反应器
——一次性全封闭生物反应器，间充质干细胞培养，种子细胞培养
美国Pall颇尔公司生产的Xpansion系列碟片式生物反应器（Xpansion多层板生物反应器，Xpansion Multiplate Bioreactor System），专为对剪切力敏感、粘附的哺乳动物细胞设计，特别适用于贴壁干细胞的培养，如间充质干细胞等，以及种子培养。通过紧凑的多层碟片结构，Xpansion生物反应器可提供高达
122,400cm2
的细胞培养面积，并提供全自动的气体扩散系统、一次性的pH、DO、温度传感器和搅拌装置，实时监控培养pH、溶氧、二氧化碳等数据，满足
cGMP
生产要求。
Xpansion碟片采用亲水聚苯乙烯材料，符合美国药典VI级标准，多个碟片叠加，形成的中央柱结构用于气体交换，并通过磁力叶轮搅拌促使培养基在各层碟片中均匀流动。
Xpansion碟片高度紧密排列，占地面积小，一个Xpansion 200生物反应器（高度仅0.8m），可提供与20个10层细胞工厂（高度约4m）相当的培养表面积，节省80%空间。而且可以从传统细胞培养耗材，直接转换为Xpansion进行培养，工艺转换简单。
Xpansion生物反应器优势
①符合美国药典VI级的原材料
②一次性使用的封闭式生物反应器，降低污染风险
③与传统培养器皿相似的化学性质表面，可轻松适应培养体系的转换
④对培养条件实时监控，提高实验重复性，可追溯，满足批次间一致性、合规性
⑤二维多层设计，培养板碟片层间距纤薄，极大程度节约空间
⑥减少操作，流程简单
⑦多种型号，满足不同工艺尺寸和扩大规模
引用文献
1. Scale-up of hepatic progenitor cells from multitray stack to 2-D bioreactors. Matthieu Egloff, Florence Collignon, Jean-Franois Michiels, et al. Springer, Article number: P61 (2013)
2. Utilizing a Multiplate Bioreactor to Preserve the Integrity of the Culture. Jean-Franois Michiels and Matthieu Egloff. Genetic Engineering & Biotechnology News, 2013, VOL.33, NO.2
3. A new cell therapy sector arising from the convergence of cell and gene therapy. E.J. Culme-Seymour, S. Edwards-Parton, J. Carmen, et al.Cytotherapy, 2013, Vol 15, Iss 4
4. IGF-1 and HGF Preconditioning of Bone Marrow Mesenchymal Stem Cells Reduced Proteinuria in Rat Focal Segmental Glomerulosclerosis Model.A. O.B.Sahan, I.Onbasilar, K.S.Gucer, et al. Cytotherapy, 2020, Volume 22, Issue 5, Page S100
5. Assessment of Pall's Xpansion bioreactor for the production of mesenchymal stromal cells for therapeutic use. H.Rouard, J.Kadoch, M.Lecuyer, et al. Cytotherapy, 2020, Volume 22, Issue 5, Pages S99-S100
6. Inverse modeling of geochemical and mechanical compaction in sedimentary basins through Polynomial Chaos Expansion. G. Porta, L. Tamellini, V. Lever, M. Riva. Water Resources Research, 2014, Volume 50, Issue 12, Pages 9414-9431
7. Pall Xpansion Bioreactor Supports Progenitor Cell Growth to >1 Million cells/cm2 and Proper Cell Differentiation. A.Laskowski, S.Gupta. Cytotherapy, 2020, Volume 22, Issue 5, Page S113
8. Overcoming adherent seed train biomass limitations: Pall Xpansion bioreactor. M.Olson, L.Truong, O.Becheau, T.Sanderson. Cytotherapy, 2020, Volume 22, Issue 5, Pages S185-S186
9. Platform approach for the AAV purification process including membrane chromatography for polishing. K.Boenning, J.Huato, A.Hejmowski, et al. Cytotherapy, 2020, Volume 22, Issue 5, Page S186
10. A rapid and reliable qPCR based system to quantitate HEK 293 residual DNA for GMP lot release in gene therapy manufacturing. L.Yan, M.Mateling, K.Norman. Cytotherapy, 2020, Volume 22, Issue 5, Page S186
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——Pall一级代理商，北京泽平